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. 2009 Jan 16;284(3):1790–1802. doi: 10.1074/jbc.M805991200

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Copyright © 2009, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — HPS gene deficiencies selectively affect the composition of cross-linked AP-3 complexes. Skin mouse fibroblast isolated from wild type C57B and HPS gene-deficient mice were incubated in the absence (n lanes) or presence (n′ lanes) of DSP. Triton-soluble supernatants were immunoprecipitated (IP) with δ AP-3 antibodies. Immunocomplexes were resolved by SDS-PAGE and analyzed by immunoblot (IB) with antibodies indicated to the left of the figure. Two different cells lines of wild type and HPS deficiencies were used in A and B, and they are numbered at the bottom of the figure. A depicts experiments performed with BLOC-1- (Pldn^pa^/^pa; lanes 3-4′) and AP-3 (Ap3b1^pe^/^pe; lanes 5-6′)-deficient cells. Note that Ap3b1^pe^/^pe corresponds to an AP-3 hypomorph phenotype. B depicts deficiencies in BLOC-2 (Hps3^coa^/^coa; lanes 3-4′) or BLOC-3 (Hps1^ep^/^ep; lanes 5-6′). C depicts BLOC-1 (Pldn^pa^/^pa; lanes 2 and 2′), BLOC-1 and -2 (Pldn^pa^/^pa, Hps6^ru^/^ru^; lanes 3 and 3′), BLOC-1, -2, and -3 (Pldn^pa^/^pa, Hps5^ru2J^/^ru2J, Hps1^ep^/^ep lanes 4 and 4′), or BLOC-2 and -3 (Hps4^le^/^le, Hps3^coa^/^coa; lanes 5 and 5′) deficiencies.