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RecFR-dsDNA interaction in the presence of ATP analogs measured with the fluorescence polarization assay. The assay was performed similarly to Fig. 2. 20 nm dsDNA was titrated with different RecF concentrations in the presence of 36 μm RecR and 2 mm of the following nucleotides: ATP (solid line), ATPγS (long dashed line), AMPPNP (short dashed line), and ADP (dotted line).
