Estimation of RecF-RecR stoichiometry using DNA binding fluorescence
polarization assay. A, solutions with five different RecF and
dsDNA concentrations were titrated with RecR. Data for 1μm RecF
and dsDNA are shown by black diamonds, for 1.5 μm, by
gray downward triangles, for 2 μm, by gray upright
triangles, for 3μm, by gray squares, for
4μm, by black circles. The curves represent
theoretical fitting calculated with SigmaPlot. B, concentrations of
RecR necessary for half saturation of the RecF-dsDNA complex were plotted
against the RecF concentrations and fitted by linear approximation.