TABLE 2.

Plasma folate in Mthfd1^gt/+ mice after 5 weeks on diet

Differences between genotypes and diets were analyzed by Student's t-test. Genotype × diet effects were analyzed by two-way ANOVA using Tukey's HSD post-hoc analysis. Data are presented as the mean ± S.E. values. p values ≤ 0.05 were considered significantly different (n = 3 per group).

Mthfd1 genotype	Diet	Plasma
		ng/ml
Mthfd1+/+	AIN-93G (C)	38.2 ± 2.57
	AIN-93G (FCD)	24.1 ± 8.90
Mthfd1gt/+	AIN-93G (C)	42.7 ± 1.65
	AIN-93G (FCD)	16.9 ± 5.11
p value, diet effect		0.006
p value, genotype effect		nsa
p value, diet × genotype effect		ns

^ans, not significant.