TABLE 4.

Colon AdoMet, AdoHcy, AdoMet/AdoHcy and uracil in nuclear DNA in Mthfd1^gt/+ mice after 5 weeks on diet

Differences between genotypes and diets were analyzed by Student's t-test. Genotype × diet effects were analyzed by two-way ANOVA using Tukey's HSD post-hoc analysis. Data are presented as the mean ± S.E. values. p values ≤ 0.05 were considered significantly different (n = 6-10 per group).

Mthfd1 genotype	Diet	AdoMet	AdoHcy	AdoMet/AdoHcy	Uracil DNA content
		pmol/μg protein				pg/μg DNA
Mthfd1+/+	AIN-93G (C)	0.2 ± 0.06	0.4 ± 0.04	0.4 ± 0.07	0.2 ± 0.02
	AIN93G (FCD)	0.2 ± 0.01	0.3 ± 0.02	0.7 ± 0.06	0.5 ± 0.10
Mthfd1gt/+	AIN-93G (C)	0.1 ± 0.02	0.4 ± 0.03	0.3 ± 0.04	0.1 ± 0.02
	AIN93G (FCD)	0.2 ± 0.02	0.3 ± 0.03	0.7 ± 0.08	0.2 ± 0.02
p value, diet effect		0.02	<0.0005	<0.0001	<0.0001
p value, genotype effect		nsa	ns	ns	<0.0001
p value, diet × genotype effect		ns	ns	ns	ns

^ans, not significant.