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. 2009 Jan 16;284(3):1533–1539. doi: 10.1074/jbc.M808281200

TABLE 5.

Metabolic profile of plasma in Mthfd1gt/+ mice after 5 weeks on diet

Differences between diets and genotypes were analyzed by Student's t-test. Genotype × diet effects were analyzed by two-way ANOVA using Tukey's HSD post-hoc analysis. Data are presented as the mean ± S.E. values. p values ≤ 0.05 were considered significantly different (n = 6 per group).

Metabolite
Mthfd1+/+
Mthfd1gt/+
p value of model effect
AIN-93G (C) AIN-93G (FCD) AIN-93G (C) AIN-93G (FCD) Diet Genotype Diet × genotype
Homocysteine (μm) 5.0 ± 0.5 5.8 ± 0.8 5.3 ± 0.3 7.1 ± 0.3 0.02 nsa ns
Cystathionine (nm) 1159 ± 103 956 ± 118 789 ± 58 1042 ± 67 ns ns 0.02b
Cysteine (μm) 200 ± 16 219 ± 17 227 ± 9 222 ± 16 ns ns ns
α-Aminobutyric acid (μm) 3.6 ± 0.3 5.9 ± 0.9 5.6 ± 0.3 5.8 ± 0.3 0.03 ns 0.07c
Methionine (μm) 40.3 ± 4.3 36.6 ± 3.6 39.9 ± 3.9 44.7 ± 1.8 ns ns ns
Glycine (μm) 341 ± 24 275 ± 19 308 ± 18 297 ± 7 0.04 ns ns
Serine (μm) 150 ± 14 138 ± 10 151 ± 15 142 ± 3 ns ns ns
Dimethylglycine (μm) 8.49 ± 0.55 7.43 ± 0.50 7.76 ± 0.64 7.50 ± 0.72 ns ns ns
Methylglycine (μm) 1.79 ± 0.07 1.41 ± 0.13 1.46 ± 0.07 1.44 ± 0.15 ns ns ns
a

ns, not significant.

b

Control Mthfd1+/+ is significantly different than control Mthfd1gt/+, p < 0.05, as analyzed by two-way ANOVA and Tukey's HSD post-hoc test for diet × genotype effect.

c

Control Mthfd1+/+ is significantly different than folate/choline-deficient Mthfd1+/+ and folate/choline-deficient Mthfd1gt/+, p < 0.05, as analyzed by two-way ANOVA and Tukey's HSD post-hoc test for diet × genotype effect.