TABLE 5.
Metabolic profile of plasma in Mthfd1gt/+ mice after 5 weeks on diet
Differences between diets and genotypes were analyzed by Student's t-test. Genotype × diet effects were analyzed by two-way ANOVA using Tukey's HSD post-hoc analysis. Data are presented as the mean ± S.E. values. p values ≤ 0.05 were considered significantly different (n = 6 per group).
|
Metabolite
|
Mthfd1+/+
|
Mthfd1gt/+
|
p value of model effect
|
||||
|---|---|---|---|---|---|---|---|
| AIN-93G (C) | AIN-93G (FCD) | AIN-93G (C) | AIN-93G (FCD) | Diet | Genotype | Diet × genotype | |
| Homocysteine (μm) | 5.0 ± 0.5 | 5.8 ± 0.8 | 5.3 ± 0.3 | 7.1 ± 0.3 | 0.02 | nsa | ns |
| Cystathionine (nm) | 1159 ± 103 | 956 ± 118 | 789 ± 58 | 1042 ± 67 | ns | ns | 0.02b |
| Cysteine (μm) | 200 ± 16 | 219 ± 17 | 227 ± 9 | 222 ± 16 | ns | ns | ns |
| α-Aminobutyric acid (μm) | 3.6 ± 0.3 | 5.9 ± 0.9 | 5.6 ± 0.3 | 5.8 ± 0.3 | 0.03 | ns | 0.07c |
| Methionine (μm) | 40.3 ± 4.3 | 36.6 ± 3.6 | 39.9 ± 3.9 | 44.7 ± 1.8 | ns | ns | ns |
| Glycine (μm) | 341 ± 24 | 275 ± 19 | 308 ± 18 | 297 ± 7 | 0.04 | ns | ns |
| Serine (μm) | 150 ± 14 | 138 ± 10 | 151 ± 15 | 142 ± 3 | ns | ns | ns |
| Dimethylglycine (μm) | 8.49 ± 0.55 | 7.43 ± 0.50 | 7.76 ± 0.64 | 7.50 ± 0.72 | ns | ns | ns |
| Methylglycine (μm) | 1.79 ± 0.07 | 1.41 ± 0.13 | 1.46 ± 0.07 | 1.44 ± 0.15 | ns | ns | ns |
ns, not significant.
Control Mthfd1+/+ is significantly different than control Mthfd1gt/+, p < 0.05, as analyzed by two-way ANOVA and Tukey's HSD post-hoc test for diet × genotype effect.
Control Mthfd1+/+ is significantly different than folate/choline-deficient Mthfd1+/+ and folate/choline-deficient Mthfd1gt/+, p < 0.05, as analyzed by two-way ANOVA and Tukey's HSD post-hoc test for diet × genotype effect.