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. 2009 Jan 16;284(3):1644–1651. doi: 10.1074/jbc.M807888200

FIGURE 2.

FIGURE 2.

Interferon induction, PKR, and eIF-2α phosphorylation, and caspase activity in transfected cells. HeLa cells were transfected with CsiRNA; PRNA sense (S) or antisense (AS) ssRNA 20- or 50-nt; or PsiRNA dsRNA 20- or 50-bp treated with T1 (+T1) or not; or pIpC. A, real time PCR measurement of human IFN-β induction relative to glyceraldehyde-3-phosphate dehydrogenase following RNA transfection. Total RNA was isolated at 5 h post-transfection. The means and standard deviations determined from three independent experiments are shown. B, Western blot analysis showing the phosphorylation of PKR and eIF-2α in HeLa cells at 8 h following transfection. C, caspase 3/7 activity at 8 h post-transfection, with and without IFN pretreatment for 20 h. The results are normalized to control pIpC transfection as 100%; the mean caspase 3/7 activity and standard deviations were determined from three independent experiments.