FIGURE 6.

PKR is required for maximal NF-κB activation but not IRF3 nuclear localization. A, immunofluorescence images showing IRF3 (green) and 4′,6′-diamino-2-phenylindole (DAPI, blue) staining following RNA transfection with CsiLUC20, PsiLUC50 or pIpC. The cells were fixed at 4 h (top three rows) and 8 h (bottom row) post-transfection. B, electrophoretic mobility shift analysis using nuclear extracts from treated HeLa cells, parental PKR⁺, or stable PKR^kd, incubated with a NF-κB oligonucleotide probe. C, Western blot analysis of IκB-α and phosphorylated eIF-2α using PsiLUC50, pIpC, or TNF-α transfected parental HeLa and PKR^kd cells. Extracts were prepared at the indicated time following treatment. Tubulin was used as a loading control.