TABLE 3.
Polymerase activity measured on DNA primer-templates and RNA primer-templates (see Table 1 for sequence information)
Polymerase assays contained 1 nm of the noted enzyme.
| Enzyme | Primer-template | Vmax | KM (dNTP) | Vmax/KM | Vmax/KM (DNA)/Vmax/KM (RNA) |
|---|---|---|---|---|---|
| pmol dNTP/nm enzyme/h | μm | ||||
| UL30 | DNA1 (5 μm) | 39.1 (2.0) | 9.40 (1.65) | 4.2 | 650 |
| UL30 | RNA1 (5 μm) | 73.5 (11.5) | 11,400 (2900) | 0.0065 | |
| UL30-UL42 | DNA1 (10 μm) | 1.92 (0.10) | 0.17 (0.04) | 11.3 | 26,000 |
| UL30-UL42 | RNA1 (10 μm) | 3.61 (0.64) | 8200 (2600) | 0.00044 | |
| UL30-UL42 | DNA1 (1 μm) | 0.57 (0.02) | 0.21 (0.03) | 2.7 | 10,000 |
| UL30-UL42 | RNA1 (1 μm) | 1.93 (0.53) | 7300 (3700) | 0.00026 | |
| pol α | DNA1 (5 μm) | 7.22 (0.17) | 8.18 (0.68) | 0.88 | 0.85 |
| pol α | RNA1 (5 μm) | 7.50 (0.20) | 7.20 (0.69) | 1.04 | |
| UL30 | DNA2 (5 μm) | 11.5 (0.4) | 3.84 (0.59) | 3.0 | 2200 |
| UL30 | RNA2 (5 μm) | NMa | NM | 0.00138 | |
| UL30-UL42 | DNA2 (5 μm) | 1.15 (0.04) | 0.39 (0.04) | 2.9 | 13,000 |
| UL30-UL42 | RNA2 (5 μm) | 0.41 (0.04) | 1800 (550) | 0.00023 | |
| UL30-UL42 | DNA2 (1 μm) | 0.35 (0.04) | 0.52 (0.25) | 0.67 | 4500 |
| UL30-UL42 | RNA2 (1 μm) | 0.60 (0.04) | 4000 (610) | 0.00015 | |
| pol α | DNA2 (5 μm) | 2.86 (0.10) | 3.17 (0.47) | 0.90 | 1.0 |
| pol α | RNA2 (5 μm) | 2.34 (0.06) | 2.62 (0.30) | 0.89 |
a
NM indicates not meaningful because the rate increased linearly with increasing dNTP concentrations.