Figure 6.

Impairment of POMC insulin signaling by palmitic acids. A–C, Hypothalamic organotypic slices expressing FoxO1GFP in POMC neurons were treated with BSA 6 h before either PBS (A) or insulin (B) (20 nm for 20 min) treatment or palmitic acid loaded on BSA (400 μm), 6 h followed by insulin treatment (20 nm for 20 min) (C). D, Quantification of FoxO1GFP nuclear translocation in control, insulin or palmitic acid followed by insulin treated organotypic slices of FoxO1GFP-POMC mice. Subcellular localization of FoxO1GFP is plotted as the percentage of neurons with cytoplasmic FoxO1GFP as in Figure 2. ***p < 0.001 compared with control; **p < 0.01 compared with insulin-treated slices, by unpaired Student's t test.