Rhes protein is significantly decreased in hemispheres of adult rats receiving 6-OHDA. (a) Western blot of Rhes protein from left and right striata of two representative rats. Lanes 1 and 2 are left and right striata, respectively, of a single rat, and lanes 3 and 4 are left and right striata, respectively, of a separate rat. (b) Western blot of TH in striata of the same rats as in (a). (c) Quantification of Western blots for Rhes protein indicating a significant decrease in Rhes protein in 6-OHDA-treated striata versus untreated striata. Data were analyzed by paired t-test; n = 8. * p<0.05.