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. 2008 Jun 25;378(1):105–117. doi: 10.1016/j.virol.2008.04.043

Fig. 2.

Fig. 2

CD8 responses to 9mer peptides of the YF envelope protein. Splenocytes of BALB/c mice immunized twice with the human 17DD YF vaccine were used for IFN-γ ELISPOT assays. The percentage of responses for each peptide was calculated according to the formula: number of spots at 1 μg/mL (or 0.1 μg/mL) × 100/number of spots at 10 μg/mL. (A) Number of IFN-γ SFC for peptides of 9 amino acids within the E57–75 sequence of the YF envelope protein; (B) number of IFN-γ SFC for the 9mers within the E329–343 sequence of the YF envelope protein; (C) IFN-γ ELISPOT evaluation of the affinity of CD8 responses to decreasing in vitro concentrations of 9mers within the E57–75 sequence of the YF envelope protein; (D) IFN-γ ELISPOT evaluation of the affinity of CD8 responses to decreasing in vitro concentration of 9mers within the E329–343 sequence of the YF envelope protein. Panels A and B represent the average of three experiments with a pool of two to three mice. Panels C and D illustrate one representative experiment out of three.