Table 2.

Results of ELISPOT, binding assay and PRED^BALB/c prediction for the CD4-positive ELISPOT 15mer peptides of the yellow fever envelope protein

A list of 94 peptides selected by PRED^BALB/c with score above 9 was compared to the CD4 ELISPOT results. The 9mers selected by the prediction software are shown below each 15mer peptide. ⁽¹⁾ Molecules of H2^d class II; ⁽²⁾ PRED^BALB/c software scores; ⁽³⁾ options of 9mer peptides within each 15mers analyzed and scored above 9 by PRED^BALB/c; ⁽⁴⁾ IC₅₀ (nmol) results for the binding assay of H2^d class II molecules; ⁎values of IC₅₀ below the cutoff of 1000 nmol, suggestive of a possible binder peptide; Shading — peptides positive for CD4 response by ELISPOT (Fig. 2C) did not predict by PRED^BALB/c above a threshold of 9.