Table 4.
Results of class II binding assay for the 15mer peptides from the yellow fever membrane protein
| Position(1) | Sequence | Binding Assay (IC50 in nmol) |
|
|---|---|---|---|
| I-Ad(2) | I-Ed(2) | ||
| M1–15 | AIDLPTHENHGLKTR | > 45,000 | 36,639 |
| M5–19 | PTHENHGLKTRQEKW | 13,183 | > 45,000 |
| M9–23 | NHGLKTRQEKWMTGR | 40,852 | > 45,000 |
| M13–27 | KTRQEKWMTGRMGER | 1903 | > 45,000 |
| M17–31 | EKWMTGRMGERQLQK | 20,419 | 20,683 |
| M21–35 | TGRMGERQLQKIERW | 42,531 | > 45,000 |
| M25–39 | GERQLQKIERWFVRN | > 45,000 | 13,242 |
| M29–43 | LQKIERWFVRNPFFA | 593⁎ | 1559 |
| M33–47 | ERWFVRNPFFAVTAL | 7.3⁎ | 2278 |
| M37–51 | VRNPFFAVTALTIAY | 40⁎ | 1917 |
| M41–55 | FFAVTALTIAYLVGS | 6213 | > 45,000 |
| M45–59 | TALTIAYLVGSNMTQ | 102⁎ | > 45,000 |
| M49–63 | IAYLVGSNMTQRVVI | 383⁎ | 29,666 |
| M53–67 | VGSNMTQRVVIALLV | 30⁎ | 32,305 |
| M57–71 | MTQRVVIALLVLAVG | 6484 | > 45,000 |
| M61–75 | VVIALLVLAVGPAYS | 380⁎ | > 45,000 |
(1) Position of each 15mer peptide in the membrane protein of the yellow fever virus; (2) molecules of H2d class II IC50 result for the binding assay for H2d class II molecules (nmol); ⁎values of IC50 below the cutoff of 1000 nmol, suggestive of a possible binder peptide.