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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Plasmid. 2008 Sep 26;60(3):200–211. doi: 10.1016/j.plasmid.2008.07.004

Figure 2.

Figure 2

IS1 integration within the 2.4 kb BamH1-BamH1 site (A region) of pSJ5.2 after transfer by tet(M) conjugative plasmid. (a) Pst1-linearized map of pSJ5.2 showing the site of IS1 insertion (open red square). Refer text for details. (b) Detail of the two overlapped IS1 copies found inserted in the pSJ5.2 derivative. Open Reading Frames and direction of transcription of the InsB and InsA genes are shown in red solid arrows. Red open square denote the formation of an IS1-beta lactamase hybrid promoter. (c) The DNA sequence of the IS1-beta-lactamase hybrid promoter in detail (Accession Number EF527806). Only the promoter region of the InsA gene from one IS1 and part of the pSJ5.2 beta-lactamase gene promoter are shown. Coordinates are in bp and relative to pSJ5.2. InsA gene and the 28 bp inverted repeat are shown in red and red italics, respectively. The beta-lactamase promoter region is shown in black. The 8 bp IS1 insertion site from pSJ5.2 is shown in blue. Black arrows denote direction of both InsA and beta-lactamase gene transcription. The -35 and -10 regions of the promoter are shown underlined. RBS (Ribosome Binding Site) in italics.