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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Plasmid. 2008 Sep 26;60(3):200–211. doi: 10.1016/j.plasmid.2008.07.004

Figure 3.

Figure 3

Copy number and distribution of the Insertion Sequence IS1 in the Neisseria gonorrhoeae and Escherichia coli strains used in the study. (a) Agarose gel electrophoresis of EcoR1-digested genomic DNA’s (Panel I) and Southern Blot hybridization with an IS1-derived non-radioactive probe (Roche, IN; Panel II). Lane Aa, Lambda + Hind III control (NEB Labs); Lane Bb, N. gonorrhoeae 15315 (showing the undigested 4.2 kb cryptic plasmid); Lane Cc, E. coli DH5α; Lane E. coli DH10B; Lane Ee, E. coli HMS174. Black arrows indicate the IS1 hybridization signals. (b) Undigested DNA from a representative E. coli HMS174 transconjugant showing the conjugative teM and pSJ5.2 both resolved (Panel I) and with at least one IS1 hybridization signal each (black arrows in Panel II).