Table 5.

List of specific primers designed

Primer name; spp. amplified	Primer sequence; primer length	Melting T; G/C%; Sec. struct.
Scripp F; all spp. in the Scrippsiella complex	GAATTGTAGTCTGGAGATGTACCAC; 25 bp	61.5 °C; 44%; weak
Scripp R	BioCTGAAGTTTTCCCCAGTTGC; 20 bp	63.5 °C; 50%; weak
Protoce F; Protoceratium reticulatum	ATGGCGAATGAAGAAGGAGT; 20 bp	62.5 °C; 45%; none
Protoce R	BioGATCCACAAGCTAAATCTGAACCT; 24 bp	63.3 °C; 41.67%; very weak
Minimum F; Prorocentrum minimum	GGGTCATGGTAGCTCGTCTA; 20 bp	61.7 °C; 55%; very weak
Minimum R	BioCGTCTTTGTGTCAGGGAAAT; 20 bp	61.5 °C; 45%; none
Rhatmicmex F; Prorocentrum rhathymum, micans and mexicanum	CTTGGCGAGATTGTCATACG; 20bp	63.2 °C; 50%; none
Rhatmicmex R	BioCGTCTATGTGTCAGGGAAGC; 20 bp	62.3 °C; 55%; none