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. Author manuscript; available in PMC: 2009 Jan 9.
Published in final edited form as: Methods Enzymol. 2008;439:181–189. doi: 10.1016/S0076-6879(07)00414-4

Figure 14.1.

Figure 14.1

Rho-associated kinase activity as determined by the immunoblotting method in different cells and tissues. (Left) Example of phosphorylation levels of MBS (pMBS) and total MBS (tMBS) in cultured endothelial cells from mouse lung (MLEC) and peripheral blood leukocytes. Positive control (PC) represents cell lysates from 3T3 fibroblasts stimulated with lysophosphatidic acid. ROCK activity is expressed as a relative blot density ratio, which is standardized to the PC blot as (pMBS sample density/tMBS PC density)/(tMBS sample density/tMBS PC density). (Right) Example of ROCK activity in tissues from mouse heart and lung, respectively.