Figure 5.
Resolving mixed population of cells. (A) HeLa cells transiently transfected with either CBG99 or CBR plasmids were counted and plated in crossing gradients on a 96-well plate. In one set of wells, the gradients were crossing each other as indicated above. In another set of wells, the identical gradient was plated alone to serve as a control. (B) Left: unfiltered bioluminescence image. Center and right: images after spectral unmixing. Boxes indicate where the gradients of CBR and CBG99 were plated.