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. Author manuscript; available in PMC: 2009 Sep 30.
Published in final edited form as: Virology. 2008 Aug 3;379(2):335–341. doi: 10.1016/j.virol.2008.06.037

Figure 4. Cyclophilin A-enhanced TRIM5α restriction occurs in early viral life cycle.

Figure 4

Time course experiment of withdrawing CsA from wild-type HIV-1 (A) and SIVagm-45/67L (B) infected rhesus macaque (SMAGI) cells. Viruses were spinoculated at 8°C for 20 minutes and transferred to a 37°C incubator to synchronize viral entry. The presence (CsA+) and absence (CsA−) of cyclosporin A (CsA) throughout the experiment was used as controls. For the withdrawal groups, CsA was added to cells when transferring plates to a 37°C incubator, and the time was set as time 0. CsA was then withdrawn at 10 minutes, 30 minutes, 1 hour, 2 hours, 4 hours, 8 hours, 12 hours, or 24 hours after time 0. Infected cells were harvested after 48 hours and analyzed by luminameter. The infectivity is presented as RLU (on a log scale).