Figure 5.

Immunoprecipitation of RecF, RecO, RecR, and Ssb protein complexes: RecF, RecO, RecR, and Ssb proteins (each at 6 μM) in indicated combinations in buffer I were incubated on ice for 30 min prior to treating with RecF antibodies. Antigen–antibody mixtures were processed essentially as described in Fig. 1. Order of addition of protein components is indicated. FORSsb indicates that all proteins were incubated together. Ssb before arrow indicates that Ssb protein was added to a preformed RecF–RecO–RecR complex. Similarly R before arrow indicates that RecR protein was added to a complex containing RecF–RecO–Ssb.