Figure 6.

RecF interaction with RecO on Ssb affinity column in the presence of ATP: RecF (38 μM) and RecO (56 μM) were mixed in buffer I containing 1 mM ATP and incubated on ice. At the end of the incubation, the sample was applied to Ssb affinity column preequilibrated in buffer I containing 1 mM ATP. Bound proteins were eluted in a linear gradient of salt as described in Fig. 5. The identity of proteins present in the peak fractions was determined by SDS/PAGE and the proteins were visualized by Coomassie blue staining (data not shown).