Figure 2.

Dose-responses of cells to 5FC. HEK 293 cells stably expressing the CD-MVP hybrid transcript were transfected with pEGFP-C1 for EGFP expression. After 24 h, the pEGFP-C1 transfected cells were incubated with E. coli BT-203 that had been transformed with vector pHippy-SspI (without siMVP library) at a bacteria-to-cell ratio (m.o.i.) of 20 at 37°C for 2 h. After washing, cells were re-fed with medium containing 25 µg/ml of gentamicin for overnight, and then incubated with 5FC at various concentrations. Photomicrographs of surviving cells showing EGFP-expressing were taken 48 h after addition of 5FC. Upper panel, GFP Images; lower panel, Optical Images.