Figure 4.

Evaluation of RNAi potency. (A) HeLa cells were transfected with chemically synthesized siMVPs at indicated concentrations with similar transfection efficiency measured as coexpressed luciferase activities. Cell lysates were subjected to western blot analysis with anti-MVP after transfection for 48 h. (B) HeLa cells were transfected with 60 ng pEGFP-MVP, 1.2 ng pRLSV40 (Rluc) and chemically synthesized siMVPs at indicated concentrations. (C) As with (B), HeLa cells were transfected with indicated concentrations of siMVP-684 derivatives synthesized in vitro. (D) As with (B) and (C), HeLa cells were transfected with indicated concentrations of siEGFPs synthesized in vitro. Results in (B), (C) and (D) were obtained from three independent experiments each performed in triplicate. RNAi potency of siMVPs was normalized by Rluc activity for transfection efficiency against unrelated siRNA control (siHB1). Data are mean ± SD.