Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Deletion of gal7 using MIRAGE to optimize DR length. (a) Expected PCR product sizes for parental and deletion alleles are indicated. Since the mechanism of cassette excision is not known, the IR is drawn with hyphenated lines. (b) Deletion of gal7 using a 25 bp DR. 1b-7b—successful precise deletion of gal7; 8b—negative control, parent. (c) Deletion of gal7 using a 20 bp DR. 1c, 7c—successful precise deletion of gal7; 2c-6c—imprecise deletion of gal7. M, DNA ladder.
