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. 2008 Nov 27;37(1):279–288. doi: 10.1093/nar/gkn927

Figure 3.

Figure 3.

Zinc is not required for UMSBP oligomerization. UMSBP, at final concentration of 7.2 µM in 20 mM Hepes pH 7.4, was treated with increasing concentrations of 1,10-o-phenanthroline, in the presence of 5 mM diamide and the reaction products were electrophoresed in a non-reducing 16.5% Tris–Tricine SDS–PAGE. The gel was stained using GelCode Blue Stain Reagent. In lane a, no diamide and no 1,10-o-phenanthroline are present. In lanes b–i: 5 mM diamide and 0, 50, 100, 200, 400, 600, 800 and 1000 µM of 1,10-o-phenanthroline, respectively. Lanes j and k represent a separate analysis: In lane j, UMSBP treated with 1000 µM of 1,10-o-phenanthroline in the absence of diamide and in lane k, UMSBP treated with 5 mM diamide in the absence of 1,10-o-phenanthroline.