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. 2008 Nov 27;37(1):279–288. doi: 10.1093/nar/gkn927

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Met¹⁶ is oxidized by H₂O₂. (A) CNBr cleavage of UMSBP was carried out as described in the Materials and methods section. UMSBP was pretreated as follows: lane 1, UMSBP was incubated in the presence of 20 mM HCl; lane 2: With 20 mM H₂O₂ under acidic conditions (as in lane 1); lane 3: neutral conditions, with no oxidant; lane 4: untreated. CNBr uncleaved UMSBP (i.e. containing Met¹⁶-O) and cleaved UMSBP (i.e. containing Met¹⁶) reaction products are designated with * and ^**, respectively. (B) and (C), MS analysis of a tryptic digest of UMSBP, treated under acidic conditions as above (B), or treated with 80 mM H₂O₂ under acidic conditions (C). Arrows indicate ion corresponding to the SAAVTCYKCGEAGHMSR sequence in UMSBP, as revealed by MS/MS analysis. Hatched arrow, denotes ion with the above sequence, but carrying Met¹⁶-sulfoxide modification instead of Met¹⁶, as revealed by MS/MS analysis. Numbers represents mass/charge ratio.