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. 2008 Nov 14;37(1):47–59. doi: 10.1093/nar/gkn901

Figure 2.

Figure 2.

Interaction regions in EV71 5′ UTR for FBP2. (A) Predicted RNA secondary structure for 5′ UTR. M-FOLD software was utilized to predict the RNA secondary structure. The first and the last nucleotides in each stem-loop are numbered as indicated. (B) Map of regions of interaction in RNA. Various truncated forms of RNA, as indicated, were transcribed in vitro and biotinylated. SF268 cell lysates were incubated with those biotin-labeled RNAs (lanes 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 and 24). Non-biotinylated RNA probes were as controls (lanes 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21 and 23). After being pulled down by streptavidin beads, the protein complex was dissolved in the SDS–PAGE as described above. Western blot was then applied to detect FBP2 in the pull-down complex.