Figure 1.

Sequence and structure of the predicted AUF1 signature motif, as identified among AUF1-associated transcripts. (A) Left, Schematic of the experimental approach. HeLa cell lysates were subjected to immunoprecipitation (IP) with either IgG or anti-AUF1 antibodies. The collections of RNAs isolated from each IP reaction were identified using microarrays [Materials and Methods section and (25)] and were used to identify a signature sequence shared by the transcripts. Right, representative AUF1 signals in IP samples, as detected by western blot analysis; Input, aliquot of lysate before IP; H.C., heavy immunoglobulin chain. (B) Probability matrix (graphic logo) of the AUF1 motif, elucidated from the array-derived experimental dataset, indicating the relative frequency with which each nucleotide is likely to be found at each position within the motif. (C) Specific sequence and secondary structure of eight representative examples of the AUF1 motif in specific mRNAs; the corresponding gene names are shown.