Figure 2.

Determination and quantification of complexes as a function of hRPA concentration. (A) ³²P-Oligo 31 (2 nM) was incubated with the indicated amounts of hRPA and then cross-linked by the addition of 0.1% glutaraldehyde for 10 min. Individual reaction mixtures were analyzed on a native 5% polycarylamide gel. (B) The total amounts of complexes (C_I + C_II + C_III, solid line), 1:1 complex (C_I, long dashed line), 2:1 complex (C_II, medium dashed line) and 3:1 complex (C_III, short dashed line) were quantified for each hRPA concentration from hRPA:Oligo 31 EMSA data (Figure 2A). The relative error is ±20%.