Figure 4.

Effect of mutations of the rasiRNA pathway genes on the presence of sense and antisense HeT-A-specific rasiRNAs in ovaries. Northern analysis of the RNA isolated from ovaries of spn-E¹/TM3, spn-E¹/spn-E^hls3987, piwi²/CyO, piwi²/piwi³, aub^QC42/CyO and aub^QC42/aub^HN flies. +/− and −/− designate hetero- and transheterozygous states of the corresponding mutations, respectively. Hybridization was done with HeT-A riboprobes from the HeT-A 3′-region to detect antisense (left part) or sense (right part) HeT-A-specific rasiRNAs. Lower panels represent hybridization with oligonucleotide complementary to the mir-13b1 microRNA or 2S RNA. P³³-labeled RNA oligonucleotides were used as size markers.