Relative formation of isoliquiritigenin metabolites by recombinant UGT enzymes (0.5 mg protein/mL). In each sample, recombinant UGT was incubated with 10 µM isoliquiritigenin at 37 °C for 20 min in the presence of UDPGA (5 mM). A) MG3 formation; B) MG4 formation; and C) MG5 formation. The control incubations contained no enzymes.
*Significant difference compared to the control as determined using one-way ANOVA with the Tukey’s test, p ≤ 0.01 (Mean ± Std. Error; N = 3). All data were normalized to the yield of MG5 (100%) catalyzed by UGT1A10.