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Computer-reconstructed mass chromatograms for the negative ion LC-MS analysis of isoliquiritigenin after incubation with pooled human kidney, human intestine, or human liver microsomes (0.5 mg protein/mL) and UDPGA. The metabolites MG3, MG4 and MG5 (also detected after incubation with human hepatocytes, see Figure 2) were identified as isoliquiritigenin 4-, 2’- and 4’-O-glucuronide, respectively. No conjugates were observed in the control incubation containing no microsomes.
