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. 2009 Jan;20(1):123–130. doi: 10.1681/ASN.2007111233

Figure 2.

Figure 2.

Proteasome-dependent processing by DC of ALB1-24 stimulates syngeneic CD8+ T cells. (A) Primary mixed leukocyte reaction (MLR). Bone marrow–derived DC from BN rats were incubated with (▒) or without ( Inline graphic) the synthetic ALB1-24 (35 μg/ml) and used to stimulate syngeneic CD8+ T cells. CD8+ T cell activation was evaluated by IFN-γ ELISPOT assay. As positive control, unpulsed DC were cultured with allogeneic LW T cells (□). (B) Secondary MLR. After 3-d primary MLR, primed CD8+ T cells were harvested and cultured again in a 2-d secondary stimulation with DC previously pulsed with or without ALB1-24. As positive control, unpulsed DC were cultured in secondary MLR with allogeneic LW T cells recovered at the end of primary MLR (□). The inhibitors EIPA (50 μM), BfA (0.5 μg/ml), or lactacystin (Lac; 10 μM), were added to DC 30 min before pulsing with ALB1-24. Data are means ± SEM (n = 5 independent experiments). *P < 0.05 versus syngeneic CD8+ T cells re-incubated with unpulsed DC or pulsed DC in presence of inhibitors.