Skip to main content
. 2009 Jan;20(1):123–130. doi: 10.1681/ASN.2007111233

Figure 5.

Figure 5.

Primary and secondary MLR in RMR rats. (A) Primary MLR. CD11c+ DC sorted from renal draining LN of vehicle or bortezomib-treated RMR rats and splenic CD11c+ DC from control rats were incubated with (▪) or without ( Inline graphic) the synthetic ALB1-24 peptide (35 μg/ml) and used to stimulate syngeneic CD8+ T cells. CD8+ T cell activation was evaluated by IFN-γ ELISPOT assay. As positive control, unpulsed DC were cultured with allogeneic LW T cells (□). (B) Secondary MLR. After 3-d primary MLR, primed CD8+ T cells were harvested and cultured in a 2-d secondary stimulation with DC previously pulsed with or without ALB1-24 peptide. As positive control, unpulsed DC were cultured in secondary MLR with allogeneic LW T cells recovered at the end of primary MLR (□). The inhibitors lactacystin (10 μM) or bortezomib (20 ng/ml) were added 30 min before peptide pulsing. Data are means ± SEM (n = 4 independent experiments). *P < 0.05 versus no ALB1-24; §P < 0.05 versus no ALB1-24 and + ALB1-24 in the presence of inhibitors.