Table 1.

Aged B cells are able to form blast cells after 48 hours.

		Media		LPS		Anti-CD40
		% Blast Formation	Fold-increase	% Blast Formation	Fold-increase	% Blast Formation	Fold-increase
Experiment 1	Young	7	__	52	8	25	4
	Aged	10	__	50	5	24	3
Experiment 2	Young	10	__	71	7	32	3
	Aged	20	__	68	3	38	2

SPC from young and aged mice were harvested and cultured for 48 hours in media only, LPS, or anti-CD40 mab with murine IL-4. Blast cells were gated as in figure 5 and percent blast cells were calculated. Percent of blasts from stimulating conditions were divided by percent of blast cells in media only to produce a fold-increase. Data is representative of 3 separate experiments.