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Schematic diagram of rDNA showing ITS1 CF and ITS1 BR annealing positions. The arrows show the annealing position for ITS1 CF, the 18S ribosomal small subunit, and ITS1 BR annealing at the 5.8S ribosomal sub-unit, to amplify the region ITS1, known to vary in size within trypanosome species. The large boxes (SSU and LSU) represent conserved coding regions, while the smaller boxes represent the spacer regions.
