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Trypanosome species and the expected band sizes on amplification with the nested ITS based primers (Cox et al, 2005)
| Trypanosome species | Expected band size from NCBI database |
| Trypanozoon members (T. brucei) | 1207 – 1224 bp |
| T. congolense Savannah | 1413 bp |
| T. congolense Kilifi | 1422 bp |
| T. congolense Forest | 1513 bp |
| T. vivax | 611 bp |
