Table 1.
Symbol of group | Actual collection days | No. of animals | Definition for purposes |
Estrous cycle | |||
E0 | day 0 | 2 | QPCR, in situ hybridization |
E7 | day 7 | 2 | QPCR |
E13 | day 13 | 2 | microarray |
Gestation | |||
Day 15 | day 15*,19, 21* | 3 | QPCR, *zymography (in situ) |
Day 21 | day 21 | 1 | in situ hybridization |
Day 25 | day 25 | 2 | microarray |
day 25, 32 | 2 | zymography (extraction) | |
Day 30 | day 27, 30, 34 | 3 | QPCR |
Day 60 | day 56, 56, 60, 64 | 4 | QPCR, microarray |
Day 150 | day 97, 148, 150 | 3 | QPCR, microarray |
day 144, 148,150 | 3 | zymography (extraction) | |
Day 250 | day 245, 249, 252 | 3 | QPCR, microarray, zymography (extraction) |
QPCR: Real-time RT-PCR. *: Each of them was used for in situ zymography as shown Day 15 and Day 21 in Figure 4.