Skip to main content
. 2008 Dec 31;6:66. doi: 10.1186/1477-7827-6-66

Table 1.

Tissue collection schedule.

Symbol of group Actual collection days No. of animals Definition for purposes
Estrous cycle
E0 day 0 2 QPCR, in situ hybridization
E7 day 7 2 QPCR
E13 day 13 2 microarray
Gestation
Day 15 day 15*,19, 21* 3 QPCR, *zymography (in situ)
Day 21 day 21 1 in situ hybridization
Day 25 day 25 2 microarray
day 25, 32 2 zymography (extraction)
Day 30 day 27, 30, 34 3 QPCR
Day 60 day 56, 56, 60, 64 4 QPCR, microarray
Day 150 day 97, 148, 150 3 QPCR, microarray
day 144, 148,150 3 zymography (extraction)
Day 250 day 245, 249, 252 3 QPCR, microarray, zymography (extraction)

QPCR: Real-time RT-PCR. *: Each of them was used for in situ zymography as shown Day 15 and Day 21 in Figure 4.