Table 4.

Codon usages and amino acid frequencies per 1000 of E. sibiricum 255-15 genome and alpha-amylase proteins (Exig_1739 and Exig_2537)

Codon	a.a.	255-15	1739	2537			255-15	1739	2537			255-15	1739	2537			255-15	1739	2537
GCA	A	22.25	7.17	7.45	CAC	H	8.34	14.34	9.31	CCA	P	6.40	12.55	1.86	TCG	S	14.80	10.75	13.04
GCC	A	22.34	17.92	20.48	CAT	H	13.16	14.34	16.76	CCC	P	2.84	7.17	5.59	TCT	S	5.67	7.17	1.86
GCG	A	23.65	16.13	27.93	ATA	I	2.11	0.00	1.86	CCG	P	24.40	26.88	40.97	ACA	T	19.13	16.13	7.45
GCT	A	12.94	10.75	5.59	ATC	I	36.04	32.26	16.76	CCT	P	4.30	3.58	5.59	ACC	T	10.08	3.58	9.31
TGC	C	1.99	1.79	0.00	ATT	I	28.88	30.47	31.66	CAA	Q	24.76	14.34	20.48	ACG	T	29.17	30.47	33.52
TGT	C	3.84	0.00	1.86	AAA	K	39.13	35.84	18.62	CAG	Q	17.85	14.34	22.35	ACT	T	4.76	7.17	3.72
GAC	D	20.69	34.05	27.93	AAG	K	14.14	8.96	9.31	AGA	R	1.97	5.38	0.00	GTA	V	7.26	10.75	5.59
GAT	D	32.71	50.18	48.42	CTA	L	3.05	1.79	1.86	AGG	R	0.59	0.00	1.86	GTC	V	41.07	26.88	42.83
GAA	E	53.26	64.52	44.69	CTC	L	19.68	7.17	5.59	CGA	R	6.12	5.38	9.31	GTG	V	10.37	5.38	9.31
GAG	E	17.44	21.51	27.93	CTG	L	22.47	21.51	27.93	CGC	R	9.79	8.96	11.17	GTT	V	14.77	12.55	9.31
TTC	F	22.14	14.34	14.90	CTT	L	16.08	8.96	3.72	CGG	R	17.76	17.92	31.66	TGG	W	9.70	30.47	31.66
TTT	F	21.48	17.92	20.48	TTA	L	20.34	17.92	20.48	CGT	R	16.41	10.75	11.17	TAC	Y	13.81	25.09	26.07
GGA	G	22.99	26.88	22.35	TTG	L	22.53	17.92	22.35	AGC	S	6.96	5.38	5.59	TAT	Y	18.94	37.63	35.38
GGC	G	14.35	5.38	13.04	ATG	M	25.80	23.30	31.66	AGT	S	9.57	10.75	9.31	TAA	*	2.02	1.79	1.86
GGG	G	10.23	16.13	13.04	AAC	N	16.91	26.88	18.62	TCA	S	10.86	10.75	9.31	TAG	*	0.13	0.00	0.00
GGT	G	21.50	17.92	11.17	AAT	N	15.59	26.88	24.21	TCC	S	8.36	8.96	14.90	TGA	*	1.36	0.00	0.00

The Chi-square results for the codon usage were not statistically significant for any of the two proteins compared to the genome.

a.a. = amino acid