Table 3.

Plasma parameters of recipient mice upon sacrifice

	n	Cholesterol (mmol/L)	Triacylglyerols (mmol/L)	NEFAs (mEq/L)	Glucose (mmol/L)	Insulin (ng/ml)	Leptin (ng/ml)
LF
MθTLR4+/+	8	23.0 ± 1.2	2.5 ± 0.4	1.42 ± 0.18	3.5 ± 0.3	0.26 ± 0.05	10 ± 2
MθTLR4-/-	6	21.0 ± 1.2	2.1 ± 0.4	1.42 ± 0.18	3.9 ± 0.4	0.21 ± 0.04	7 ± 1
HF
MθTLR4+/+	8	17.9 ± 2.6	2.5 ± 0.3	1.36 ± 0.13	3.6 ± 0.3	0.25 ± 0.04	23 ± 5
MθTLR4-/-	5	20.0 ± 2.1	2.3 ± 0.1	1.47 ± 0.17	4.2 ± 0.3	0.21 ± 0.03	24 ± 1
HFSFA
MθTLR4+/+	10	18.6 ± 1.1	2.7 ± 0.2	1.00 ± 0.05†	5.2 ± 0,3†	0.55 ± 0.10††	26 ± 3*
MθTLR4-/-	6	18.7 ± 1.2	2.5 ± 0.2	1.08 ± 0.03†	5.9 ± 1.2†	0.51 ± 0.10††	30 ± 3*
HFSFA+FO
MθTLR4+/+	9	13.6 ± 1.4‡	2.0 ± 0.2	1.04 ± 0.04†	5.5 ± 0.4††	0.68 ± 0.11††	54 ± 11‡
MθTLR4-/-	6	15.4 ± 1.9‡	2.1 ± 0.3	1.01 ± 0.09†	6.5 ± 0.4††	0.56 ± 0.08††	64 ± 6‡

Data are mean ± SEM from 5-11 mice per group. Plasma parameters were measured after recipient mice were fed their respective diets for 12 wks. Mice were fasted overnight before sacrifice and plasma collection. Student’s t-tests performed using Prism Graphpad revealed no significant differences among MθTLR4+/+ and MθTLR4-/- groups for each diet. Two-way ANOVA revealed main effect for diet for cholesterol (P<0.001), NEFA (P<0.001), glucose (P<0.0001), insulin (P<0.0001), and leptin (P<0.00001). Main effects for macrophage TLR4 genotype reached near significance for glucose (P=0.07) Significant findings of post-hoc tests as indicated.

^*P<0.05 for diet compared to LF

^†P<0.05 for diet compared to LF and HF

^††P<0.01 for diet compared to LF and HF

^‡P<0.00001 for diet compared to all other diets