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Soluble guanylyl cyclase activation by NOS. Intracellular cGMP levels in 106 RFL-6 detector cells were measured under basal conditions and in the presence of either 10 μM sodium nitroprusside (SNP), a ·NO donor compound, or 3 μg of purified rat NOS-I (incubated for 10 min at 37°C in the presence of 1 mM isobutylmethylxanthine. Mean ± SEM (n = 3).
