Figure 3.

Dependence of the ·NO signal derived from NOS on H₄Bip, Arg, and SOD. CaM was added at 0 min. (A) ·NO formation from CaM-activated NOS (30 μg) expressed in mV of the electrode signal either in the presence of 35.7 μM SOD, with (○) or without (▪) 10 μM H₄Bip, or in the absence of SOD (•), when no signal was observed, irrespective of whether H₄Bip was present. Tracings are representative of six experiments with similar results. (B) ·NO formation from NOS (1.7 μg) in the presence of 35.7 μM SOD and abolition upon addition of 1 mM N^ω-nitro-l-arginine (NO₂Arg). (C) Dependence of ·NO formation from NOS (0.7 μg) on time of SOD addition, which was at 0 (○) or 12 min (•).