SOD dependence of ·NO detection. NOS
(○, □, up to 24 μg), the NO donor SperNO
(•, 40 μM), and the NO− donor Angeli’s salt
(▴, 1 μM; ▪, 10 μM) were incubated with
different SOD concentrations in the absence (□,
▪, ▴) or presence (○, •) of
10 μM H4Bip and otherwise standard assay conditions
(i.e., with Arg). ·NO was detected electrochemically
(A) or by chemiluminescence (B);
n = 3.