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NADPH consumption by ONOO−. Recovery of NADPH was determined spectrophotometrically under NOS assay conditions in the presence of increasing concentrations of ONOO−. (A) Stoichiometric loss of NADPH (100 μM) by ONOO− corrected for autoxidation of NADPH. (B) Protection of NADPH (25 μM final concentration) from ONOO− (12.5 μM)-induced loss in the presence of increasing concentrations of 2-mercaptoethanol.
