Table 1.
Arg-derived products from NOS
| Product | GSH, mM | n | Amount, % of Cit |
|---|---|---|---|
| Cit | 7 | 3 | 100 ± 2.3 |
| HbO2/Hb+ assay | 7 | 3 | 87.5 ± 0.9 |
| NOx− | 0 | 3 | 68.3 ± 2.1 |
| 7 | 3 | 61.2 ± 2.2 | |
| GSNO* | 2 | 8 | 5.0 ± 1.1 |
| NH2OH* | 7 | 3 | 13.3 ± 3.5 |
| N2O* | 0 | 3 | 5.3 ± 0.9 |
| 7 | 3 | 6.9 ± 1.4 |
Purified porcine cerebellum NOS (up to 6.22 μg; specific activity 428 nmol of Cit min−1 per mg of protein) prepared in the absence of thiols was incubated under Vmax conditions in the presence of 7 mM GSH in a final volume of 0.1–1 ml at 37°C. Arg-derived products are expressed in % of Cit formation.
*
Corrected for incomplete recovery as described in the text.