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Secondary structure model of N6/C6 split permease. The lac permease is shown as the N-terminal six transmembrane helices (N6) and the C-terminal six transmembrane helices (C6). N6 has a biotin acceptor domain (BD) at the C terminus. Single Cys replacements in helix I (mutants F27C, P28C, and F29C), helix II (mutants I52C, S53C, and L54C), helix VII (mutants Q242C and N245C), and helix XI (mutant A361C) are numbered and highlighted.
