Table 1.

Purification of recombinant MBP-hFAS domain I from E. coli

Step	Total protein (mg)	Specific activities, nmol/min per mg of protein
		AT	MT	DH
Cell-free supernatant	1200	57	83	0.5
Ammonium sulfate fraction	400	11	4	0.8
Amylose-resin column	8	516	171	50.5
FPLC Superose 6	4	801	272	65.2

AT, acetyl transacylase; MT, malonyl transacylase; DH, β-hydroxyacyl dehydratase; FPLC, fast-protein liquid chromatography.