Table 2.
Comparison of recombinant hFAS and its subdomain activities with native HepG2 FAS
| Functional assays | HepG2 FAS* | MBP-hFAS | MBP-hFAS dI | TRX-hFAS dII-III | TRX-hFAS dII′-III |
|---|---|---|---|---|---|
| FAS activities | |||||
| NADPH oxidation | 462 | 611 | |||
| [1-14C]acetyl-CoA | 29.6 | 43.0 | |||
| [2-14C]malonyl-CoA | 220.6 | 300.0 | |||
| FAS partial activities | |||||
| Acetyl transacylase | 1336.0 | 800.3 | 800.5 | ||
| Malonyl transacylase | 558 | 272 | 272 | ||
| β-Ketoacyl synthase | 0.20 | 0.14 | ND | ||
| β-Hydroxyacyl dehydratase | 57 | 49 | 65 | ||
| β-Ketoacyl reductase | 2514 | 1921 | 2754 | ||
| Enoyl reductase | 89.3 | 34.4 | 95.2 | ||
| Thioesterase | 54.8 | 41.9 | 50.7 | 160.0 |
The assays used to measure the overall and partial activities of hFAS were performed as described in Materials and Methods. The activities were expressed as nmol/min/mg of protein. ND, not determined.
*
The values given are from our previously published paper (5).